That was exciting, now wasn't it?

The backwards-traced model of cellular retinoic-acid-binding protein is discussed in: Kleywegt, G.J. and Jones, T.A. (1995). Where freedom is given, liberties are taken. Structure 3, 535-540 (PubMed).

If you want, you can read the full story on 2CHR versus 1CHR in: Kleywegt, G.J., Hoier, H. and Jones, T.A. (1996). A re-evaluation of the crystal structure of chloromuconate cycloisomerase. Acta Crystallogr D52, 858-863. (PubMed).

Validation of CA-only models (including 1PTE and 1PHY) is described in: Kleywegt, G.J. (1997). Validation of protein models from Calpha coordinates alone. J Mol Biol 273(2), 371-376 (PubMed).

The debate about the subject of the exercise "BoTox or NoTox" has now been settled by a high-resolution structure solved by a third group (PubMed) (PDB entry 1XTG) (EDS).

During this practical you may have gotten the impression that most protein models are wrong or at least seriously flawed. This is of course not the case - the large majority of models are quite acceptable (although small errors may remain even in high-resolution structures, and although even at high resolution there are still subjective choices involved in the model-building and refinement process). However, for teaching purposes poor models are more interesting than essentially correct ones!

All there is left to do now is to compare your answers to mine!

Latest update on 12 December, 2008.