• Protein family: WW
• Protein number: 2
• Peptide number: 2

Protein:

• Protein name: Nedd4
• Protein source: Rattus Norvegicus

Structural information:

• PDB apo: 1i5h
• Experimental technique apo: NMR, 15 structures
• Resolution apo (Å):
• PDB complex: 1i5h
• Experimental technique complex:
• Resolution complex (Å):

Peptide:

• Name of peptide (in paper): Pintide
• Phosphorylated residue type: Ser
• Origin protein of peptide: Synthetic
• Source of origin protein: Synthetic
• Phosphorylated residue number in origin protein: 0
• First position before phosphorylated residue: -3
• Last position before phosphorylated residue: 4
• Sequence of peptide: WFYSPFLE
• N-terminus: Fluorescein
• C-terminus: Free
• Peptide length: 8

Mutational information:

• wt or mutant peptide: mutant
• Position of mutation in peptide: 0
• Mutated residue in peptide: pS
• New residue in peptide: S
• wt or mutant protein: wt
• Position of mutation in protein:
• Mutated residue in protein:
• New residue in protein:

Thermodynamic information:

• Kdiss (mM):
• Km (M):
• IC50 (M):
• DGdiss (kJ/mol), experimental:
• DGdiss (kJ/mol), calculated with FOLDx:
• DDGdiss (kJ/mol):

Experimental conditions:

• Method: Fluorescence polarization
• Temperature ºC: 22
• pH: 7.4
• Buffer conditions: 50 mM Hepes, 100 mM NaCl, 2% Glycerol

Bibliography:

• Reference: Lu et al. 1999 Science 283, 1325
• MedLine_ID: 10037602