• Protein family: SH2
• Protein number: 8
• Peptide number: 69

Protein:

• Protein name: Grb2
• Protein source: Homo Sapiens

Structural information:

• PDB apo: 1jyu
• Experimental technique apo: X-ray
• Resolution apo (Å): 2.75
• PDB complex: 1qg1
• Experimental technique complex: NMR, min. av. Str.
• Resolution complex (Å):

Peptide:

• Name of peptide (in paper): pYVNV
• Phosphorylated residue type: Tyr
• Origin protein of peptide: shc
• Source of origin protein: Homo Sapiens
• Phosphorylated residue number in origin protein: 317
• First position before phosphorylated residue: -3
• Last position before phosphorylated residue: 8
• Sequence of peptide: DPSpYVNVQNLDK
• N-terminus: Free
• C-terminus: Free
• Peptide length: 12

Mutational information:

• wt or mutant peptide: wt
• Position of mutation in peptide:
• Mutated residue in peptide:
• New residue in peptide:
• wt or mutant protein: wt
• Position of mutation in protein:
• Mutated residue in protein:
• New residue in protein:

Thermodynamic information:

• Kdiss (mM): 0.012
• Km (M):
• IC50 (M):
• DGdiss (kJ/mol), experimental: -44.44
• DGdiss (kJ/mol), calculated with FOLDx:
• DDGdiss (kJ/mol):

Experimental conditions:

• Method: SPR
• Temperature ºC: 20
• pH: 7.5
• Buffer conditions: 50 mM Hepes, 150 mM NaCl, 3 mM DTT

Bibliography:

• Reference: Marengere et al. 1994 Nature 369, 502
• MedLine_ID: 7515480