• Protein family: SH2
• Protein number: 6
• Peptide number: 4

Protein:

• Protein name: Fyn
• Protein source: Homo Sapiens

Structural information:

• PDB apo: 1g83
• Experimental technique apo: X-ray
• Resolution apo (Å): 2.6
• PDB complex: 1aou
• Experimental technique complex: NMR, 22 structures
• Resolution complex (Å):

Peptide:

• Name of peptide (in paper): Ile peptide
• Phosphorylated residue type: Tyr
• Origin protein of peptide: middle T
• Source of origin protein: Polyoma Virus
• Phosphorylated residue number in origin protein: 324
• First position before phosphorylated residue: -3
• Last position before phosphorylated residue: 7
• Sequence of peptide: EPQpYEEIPIYL
• N-terminus: Free
• C-terminus: Free
• Peptide length: 11

Mutational information:

• wt or mutant peptide: wt
• Position of mutation in peptide:
• Mutated residue in peptide:
• New residue in peptide:
• wt or mutant protein: wt
• Position of mutation in protein:
• Mutated residue in protein:
• New residue in protein:

Thermodynamic information:

• Kdiss (mM): 0.068
• Km (M):
• IC50 (M):
• DGdiss (kJ/mol), experimental: -40.9
• DGdiss (kJ/mol), calculated with FOLDx:
• DDGdiss (kJ/mol):

Experimental conditions:

• Method: ITC
• Temperature ºC: 25
• pH: 6
• Buffer conditions: 20 mM Mes, 1 mM DTT, 50 mM NaCl

Bibliography:

• Reference: Chung et al. 1999 ProtSci 8, 1962
• MedLine_ID: 10548041