• Protein family: Polo Box
• Protein number: 1
• Peptide number: 6

Protein:

• Protein name: Plk1
• Protein source: Homo Sapiens

Structural information:

• PDB apo: 1q4o
• Experimental technique apo: X-ray
• Resolution apo (Å): 2.2
• PDB complex: 1umw
• Experimental technique complex: X-ray
• Resolution complex (Å): 1.9

Peptide:

• Name of peptide (in paper): PoloBoxtide-Optimal
• Phosphorylated residue type: Thr
• Origin protein of peptide: Synthetic
• Source of origin protein: Synthetic
• Phosphorylated residue number in origin protein: 0
• First position before phosphorylated residue: -7
• Last position before phosphorylated residue: 7
• Sequence of peptide: MAGPMQSpTPLNGAKK
• N-terminus: Free
• C-terminus: Free
• Peptide length: 15

Mutational information:

• wt or mutant peptide: wt
• Position of mutation in peptide:
• Mutated residue in peptide:
• New residue in peptide:
• wt or mutant protein: mutant
• Position of mutation in protein: 414
• Mutated residue in protein: W
• New residue in protein: F

Thermodynamic information:

• Kdiss (mM): 100
• Km (M):
• IC50 (M):
• DGdiss (kJ/mol), experimental: -22.44
• DGdiss (kJ/mol), calculated with FOLDx:
• DDGdiss (kJ/mol): 14.33

Experimental conditions:

• Method: ITC
• Temperature ºC:
• pH:
• Buffer conditions:

Bibliography:

• Reference: Elia et al. 2003 Cell 115, 83
• MedLine_ID: 14532005