• Protein family: Phosphoserine phosphatase
• Protein number: 1
• Peptide number: 1

Protein:

• Protein name: Cdc14B
• Protein source: Homo Sapiens

Structural information:

• PDB apo: 1ohc
• Experimental technique apo: X-ray
• Resolution apo (Å): 2.5
• PDB complex: 1ohe
• Experimental technique complex: X-ray
• Resolution complex (Å): 2.2

Peptide:

• Name of peptide (in paper): Optimal phosphopeptide
• Phosphorylated residue type: Ser
• Origin protein of peptide: Synthetic
• Source of origin protein: Synthetic
• Phosphorylated residue number in origin protein:
• First position before phosphorylated residue: -1
• Last position before phosphorylated residue: 4
• Sequence of peptide: ApSPRRR
• N-terminus: Acetylated
• C-terminus: Amidated
• Peptide length: 6

Mutational information:

• wt or mutant peptide: wt
• Position of mutation in peptide:
• Mutated residue in peptide:
• New residue in peptide:
• wt or mutant protein: wt
• Position of mutation in protein:
• Mutated residue in protein:
• New residue in protein:

Thermodynamic information:

• Kdiss (mM):
• Km (M): 1.70E-04
• IC50 (M):
• DGdiss (kJ/mol), experimental: -21.15
• DGdiss (kJ/mol), calculated with FOLDx:
• DDGdiss (kJ/mol):

Experimental conditions:

• Method: Enzyme activity
• Temperature ºC: 20
• pH: 7.5
• Buffer conditions: 50 mM Tris, 750 mM NaCl, 1 mM MgCl2, 0.6 mM DTT, 0.2 mM EDTA, 1% (v/v) glycerol

Bibliography:

• Reference: Gray et al. 2003 EMBO J 22(14) 3524
• MedLine_ID: 12853468