• Protein family: Histone acetyltransferase
• Protein number: 1
• Peptide number: 6

Protein:

• Protein name: GCN5 R164K
• Protein source: Tetrahymena thermophila

Structural information:

• PDB apo: 1qsr
• Experimental technique apo: X-ray
• Resolution apo (Å): 2
• PDB complex: 1pua
• Experimental technique complex: X-ray
• Resolution complex (Å): 2.3

Peptide:

• Name of peptide (in paper): phos-H3
• Phosphorylated residue type: Ser
• Origin protein of peptide: Histone3
• Source of origin protein: Saccharomyces Cerevisiae
• Phosphorylated residue number in origin protein: 10
• First position before phosphorylated residue: -9
• Last position before phosphorylated residue: 10
• Sequence of peptide: ARTKQTARKpSTGGKAPRKQL
• N-terminus: Free
• C-terminus: Free
• Peptide length: 20

Mutational information:

• wt or mutant peptide: wt
• Position of mutation in peptide:
• Mutated residue in peptide:
• New residue in peptide:
• wt or mutant protein: mutant
• Position of mutation in protein: 164
• Mutated residue in protein: R
• New residue in protein: K

Thermodynamic information:

• Kdiss (mM):
• Km (M): 4.20E-04
• IC50 (M):
• DGdiss (kJ/mol), experimental: -19.08
• DGdiss (kJ/mol), calculated with FOLDx:
• DDGdiss (kJ/mol): 3.67

Experimental conditions:

• Method: Enzyme activity
• Temperature ºC: 22
• pH: 7.5
• Buffer conditions: 100 mM Acetate, 50 mM Tris, 50 mM Bis-tris

Bibliography:

• Reference: Cheung et al. 2000 MolCell 5, 905
• MedLine_ID: 10911985