• Protein family: FHA
• Protein number: 2
• Peptide number: 27

Protein:

• Protein name: Rad53 FHA2
• Protein source: Saccharomyces Cerevisiae

Structural information:

• PDB apo: 1fhq
• Experimental technique apo: NMR, 20 structures
• Resolution apo (Å):
• PDB complex: 1k2m
• Experimental technique complex: NMR, 22 structures
• Resolution complex (Å):

Peptide:

• Name of peptide (in paper): Rad9-1
• Phosphorylated residue type: Tyr
• Origin protein of peptide: Rad9
• Source of origin protein: Saccharomyces Cerevisiae
• Phosphorylated residue number in origin protein: 829
• First position before phosphorylated residue: -3
• Last position before phosphorylated residue: 3
• Sequence of peptide: EDIpYYLD
• N-terminus: Free
• C-terminus: Free
• Peptide length: 7

Mutational information:

• wt or mutant peptide: wt
• Position of mutation in peptide:
• Mutated residue in peptide:
• New residue in peptide:
• wt or mutant protein: mutant
• Position of mutation in protein: 655/656/657
• Mutated residue in protein: N/V/S
• New residue in protein: A/A/A

Thermodynamic information:

• Kdiss (mM): 1000
• Km (M):
• IC50 (M):
• DGdiss (kJ/mol), experimental: -16.83
• DGdiss (kJ/mol), calculated with FOLDx:
• DDGdiss (kJ/mol): 5.61

Experimental conditions:

• Method: NMR
• Temperature ºC: 20
• pH: 6.5
• Buffer conditions: 10 mM Phosphate, 1 mM DTT, 1 mM EDTA

Bibliography:

• Reference: Liao et al. 1999 JMB 294, 1041
• MedLine_ID: 10588905